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Abstract
Grant Number: 2R01LM005652-14 Project Title: Annotating Functional Sites in 3D Biological Structures
PI Information: Name Title ALTMAN, RUSS BIAGIO. russ.altman@stanford.edu PROFESSOR Abstract: DESCRIPTION (provided by applicant): Project Summary/Abstract Dramatic advances in our understanding of molecular structure and function promise to accelerate the creation of new diagnostics and therapeutics. However the link between the structure of a biological macromolecule and its function is usually not obvious: fundamental to understanding how a molecule functions is an understanding of how its structure behaves over time. Recent advances in molecular dynamics simulations now allow the rapid collection of information about structural motion. These data sets are huge, and require statistical machine learning algorithms to characterize and recognize patterns relevant to function. The National Library of Medicine's new long-range plan calls for research in the use of advanced simulation and machine learning algorithms in support of biomedical research. This proposal focuses on annotating molecular structures with missing or incomplete functional information. We are particularly interested in identifying binding sites and active sites in proteins. We bring together simulation and machine learning, and hypothesize that the performance of structure- based function annotation methods will dramatically improve with the addition of information about dynamics. Thus, our specific aims are (1) to develop methods for recognizing function from structural dynamics and diversity, (2) to develop capabilities for large scale clustering and analysis tools for the discovery of novel functions, and (3) to apply our tools to challenging and important biological systems, while disseminating our software, data and capabilities to the biomedical research community. In particular, we will focus our new capabilities on three difficult function annotation challenges: ATP binding sites, phosphorylation sites, and metabolizing enzyme active sites.
Public Health Relevance: Project Narrative The explosion in data related to molecular biology has created great opportunities for new disease diagnostics and therapies. One source of data is the three-dimensional (3D) structure of biological molecules such as proteins, DNA and RNA. This work focuses on using computational technologies to understand how these structures perform their function, so we have a better understanding of both normal and disease processes.
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Institution: STANFORD UNIVERSITY STANFORD, CA 94305 Fiscal Year: 2009 Department: GENETICS Project Start: 01-JUL-1994 Project End: 31-AUG-2014 ICD: NATIONAL LIBRARY OF MEDICINE IRG: BLR