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Abstract
Grant Number: 5R44NS048734-03 Project Title: Salmon fibrin gels for surgical applications
PI Information: Name Title SAWYER, EVELYN S. esawyer@searunholdings.com Abstract: DESCRIPTION (provided by applicant): Our objective is to develop a new biomaterial, a salmon-derived fibrin gel, which will bridge injured CNS tissue, support and promote axonal sprouting and growth, and deliver neurotrophic factors and cells. In Aim #1 we will test the efficacy of salmon-derived fibrin gels in animal models of CNS injury. Using the rat dorsal hemi-section, contusion, and fluid percussion models, we will compare the salmon material to human-derived fibrin gels for efficacy, inflammatory response, and possible functional changes. In Aim #2 we will seek to identify the growth-promoting factors that are involved in the salmon gels. We will determine if there is a small active molecule bound to the fibrinogen, and what properties of the fibrinogen molecule are important. In Aim #3 we plan pre-clinical studies of viral clearance, immunogenicity, and stability that are necessary for FDA approval of salmon fibrinogen and thrombin. We will spike the proteins with virus, and determine combinations of heat and gamma radiation that will achieve viral reduction, preserve protein efficacy, and not result in immunogenicity. Tissues from treated rats (Aim #1) will be examined for inflammatory response, and plasma samples taken for antibody studies. The cost of spinal cord and brain injury in both dollars and human suffering is a major public health issue. If the neurosurgeon could intervene with a material that promotes neuronal regrowth and connectivity, the impacts for the patient and for society are enormous.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
fibrin, gel, trout /salmon
antibody, biomaterial, brain injury, cell, contusion, experience, fibrinogen, fluid, gamma radiation, head, heat, hemostatic, human, injury, material, model, neuronal guidance, neurotrophic factor, plasma, protein, public health, reduction, sectioning, spinal cord, spinal cord injury, thrombin, tissue, virus
Institution: SEA RUN HOLDINGS, INC. 76 PINE ST FREEPORT, ME 04032 Fiscal Year: 2007 Department: Project Start: 15-MAY-2004 Project End: 31-DEC-2008 ICD: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE IRG: ZRG1