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Abstract

Grant Number:	5P01AI061537-020002
Project Title:	A proteomic approach to host humoral immune response

PI Information:	Name	Email	Title
	HAOPING, LIU

Abstract: Candida albicans is the most frequently isolated fungal pathogen of humans. It is a common pathogen that causes bloodstream infection in hospitals, with estimated mortality at 40%. Increased use of prophylactic antifungal drugs in high-risk patients accelerates the development of drug resistance. Alternative antifungal therapies are urgently needed. One promising approach to avoid anti-fungal resistance is to use immune-based therapies. Protective antibodies against C. albicans heat shock protein 90 (Hsp90) or beta-mannan have been shown to be active against hematogenously disseminated candidiasis in experimental mice. In particular, a human genetically recombinant antibody against the Hsp90 is currently in a multinational trial in patients with disseminated candidiasis. To identify potential vaccines and protective antibodies, we propose to develop a C. albicans protein microarray to profile humoral responses in a large number of patients with invasive candidiasis.

Public Health Relevance:
This Public Health Relevance is not available.

Thesaurus Terms:
Candida albicans, candidiasis, fungal protein, host organism interaction, humoral immunity, immune response, proteomics
antifungal agent, fungal antigen, heat shock protein, mannan, mass spectrometry, mycosis, mycotoxin, posttranslational modification, recombinant protein, serum
SDS polyacrylamide gel electrophoresis, enzyme linked immunosorbent assay, human tissue, microarray technology, polymerase chain reaction, two dimensional gel electrophoresis, western blotting

Institution:	UNIVERSITY OF CALIFORNIA IRVINE
	IRVINE, CA 926977600
Fiscal Year:	2005
Department:
Project Start:
Project End:
ICD:	NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
IRG:	ZAI1

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