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Abstract

Grant Number:	1K25GM071951-01
Project Title:	Intelligent Aids for Proteomic Data Mining

PI Information:	Name	Email	Title
	GOPALAKRISHNAN, VANATHI	vanathi@cbmi.pitt.edu

Abstract: DESCRIPTION (provided by applicant): Primary purpose of this proposal is to provide the applicant with the means and structures for achieving two goals; (1) to develop intelligent computational aids for mining proteomic data accumulating from high throughput techniques like SELDI-TOF mass spectrometry; and (2) the long-term goal is to gain independence as a biomedical informatics researcher by developing methodological expertise in Bayesian methods and proteomic technologies. Applicant will obtain further instruction in probabilistic methods of data analysis; and she will receive education on proteomic technologies that are driving today's proteome research. Training will be provided through formal coursework, directed readings, seminars and conferences in addition to research directed by excellent mentors. Applicant's research project involves a novel combination of techniques for use in proteomic data analysis. Previous research has included the use of techniques such as genetic algorithms and neural networks for analysis of proteomic data. These techniques were not explicitly designed to take into account background and prior knowledge. Hypothesis of this project is that background knowledge and machine learning techniques can positively influence the selection of appropriate biomarkers from proteomic data, enabling efficient and accurate analysis of massive datasets arising from proteomic profiling studies. Therefore, this project will satisfy four aims: (1) development of a wrapper-based machine learning tool; (2) augment the tool with prior knowledge such as heuristic rules and relationships in the data; (3) use these features along with de-identified patient information as input to classification systems; and (4) evaluate existing techniques for interpreting tandem mass spectrometry (MS-MS or MS/MS) data, and propose, implement and evaluate a Bayesian method for identification of peptides and proteins indicated by the MS-MS spectrum.

Public Health Relevance:
This Public Health Relevance is not available.

Thesaurus Terms:
bioinformatics, biomarker, computer assisted sequence analysis, computer program /software, computer system design /evaluation, data management, genetic disorder diagnosis, proteomics
diagnosis design /evaluation, mass spectrometry
biotechnology, human data

Institution:	UNIVERSITY OF PITTSBURGH AT PITTSBURGH
	OFFICE OF RESEARCH
	PITTSBURGH, PA 15213
Fiscal Year:	2004
Department:	MEDICINE
Project Start:	01-JUL-2004
Project End:	30-JUN-2009
ICD:	NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
IRG:	ZRG1

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