| Version 2.5.2.0 |
|
|
Abstract
Grant Number: 5R21EB002228-02 Project Title: In Vitro Synthesis of a Microvascular Network
PI Information: Name Title TIEN, JOE Y. jtien@bu.edu ASSISTANT PROFESSOR Abstract: DESCRIPTION (provided by applicant): This two-year proposal describes the development of processes, based on microlithography, to synthesize a human dermal microvascular network in vitro. The methods developed in this work will support the following long-term goals: 1) modeling of human inflammatory responses in vitro for high-throughput screening of agents that can reverse an induced response, and 2) engineering of full-thickness skin grafts with an integrated vasculature. By allowing the synthesis of tissue with a controlled microscale architecture, these lithographic methods will complement current strategies in tissue engineering that rely on cellular self-organization induced by growth factors. The proposed schemes will arrange primary microvascular cells into a three-dimensional network of open tubes initially, and then continuously perfuse the construct to allow its maturation into a functional microcirculation. We will determine the ranges of network shear stress, luminal pressure, and oxidative stress that enable the as-synthesized construct to develop a histological organization characteristic of a human dermal microvascular bed. Moreover, we will vary perfusion conditions to tune the reactivity of the in vitro network to inflammatory cytokines, and to enhance the extravasation of neutrophils in a stimulated network. This work will further determine the thickness of tissue that can be sustained metabolically by an engineered microvascular construct comprised of two interconnected plexuses.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
artificial skin, blood vessel, capillary bed, cell component structure /function, microcirculation, tissue engineering
anatomy, angiogenesis, cytokine, fibroblast, inflammation, molecular film, oxidative stress, shear stress, skin circulation, vascular endothelium, vascular smooth muscle
bioengineering /biomedical engineering, biotechnology, confocal scanning microscopy, human tissue, immunofluorescence technique, video microscopy
Institution: BOSTON UNIVERSITY 881 COMMONWEALTH AVENUE BOSTON, MA 02215 Fiscal Year: 2004 Department: BIOMEDICAL ENGINEERING Project Start: 30-SEP-2003 Project End: 31-MAY-2007 ICD: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING IRG: ZRG1