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Abstract
Grant Number: 5R33CA097541-02 Project Title: Near IR fluorescent probes/molecular analysis of cancer
PI Information: Name Title WAGGONER, ALAN S. waggoner@andrew.cmu.edu PROFESSOR Abstract: DESCRIPTION (provided by applicant): It is well known that fluorescence detection provides the basis of flow cytometry, fluorescence imaging, DNA sequencing, microarray detection, drug testing, diagnostics, and so on. It is our goal to extend the power of fluorescence detection in cancer research and diagnostics, by developing new fluorescent probes that absorb and fluoresce in the red and near infrared regions of the spectrum. These advances will: (1) increase the number of parameters that can be acquired in a single experiment (hence, more information becomes available through correlation of cell/tissue properties), (2) increase sensitivity (since cellular autofluorescence is much smaller in the red and near IR), and (3) allow imaging deeper into tissues (since absorbance and fluorescence will be away from hemoglobin absorption and in a range where light scattering and absorption is reduced). The new fluorescent reagents will be photostable, chemically stable, water soluble, brightly fluorescent and non-photo-toxic to cells and tissues. This will be accomplished by synthetic modifications in the molecular structure of cyanine (polymethine) dyes and by stabilizing dye structure through encapsulation methods including cyanine-cyclodextrin rotaxanes, nucleic acid encapsulation, sandwich complexation, and covalent self-encapsulation.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
chemical synthesis, fluorescent dye /probe, infrared radiation, neoplasm /cancer diagnosis, technology /technique development
anthracene, chemical stability, chemical structure function, chromophore, cyanine, cyclodextrin, microcapsule, nucleic acid
flow cytometry, imaging /visualization /scanning, microscopy, tissue /cell culture
Institution: CARNEGIE-MELLON UNIVERSITY 5000 FORBES AVE PITTSBURGH, PA 15213 Fiscal Year: 2003 Department: BIOLOGICAL SCIENCES Project Start: 18-JUL-2002 Project End: 30-JUN-2005 ICD: NATIONAL CANCER INSTITUTE IRG: ZCA1