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Abstract
Grant Number: 1R21CA097511-01 Project Title: DNA Methylation in Patients with Non-Hodgkin's Lymphoma
PI Information: Name Title LEVENSON, VICTOR V. victor_levenson@rush.edu RESEARCH ASSOCIATE PROFESSOR Abstract: Technological advances of molecular biology are posed to bring new methods of diagnosis and treatment to clinical practice. Among other things these methods should improve on the sensitivity of existing techniques, be sufficiently robust to allow multi-parametric evaluation of clinical samples, and should not require invasive procedures for sample acquisition. In this proposal we will test a combination of existing modern techniques, which is designed to provide high-throughput, multi- parametric detection of diffuse large B-cell lymphoma. In R21 phase of the project we will use cultured cell and paraffin-embedded tissues of patients to determine sensitivity, selectivity and variability of the assay. When the milestones of R21 phase are met we will move into clinical R33 phase of the project, testing the assay's ability to detect lymphoma- specific DNA in patient's plasma. We will test correlation of the assay's results with clinical diagnosis of diffuse large B-cell lymphoma, and with clinical outcomes, including disease-free survival and drug resistence. When the assay is optimized for disease detection in patient's plasma we will also address the issue of residual disease detection and monitoring of treatment efficacy.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
B cell lymphoma, DNA methylation, diagnosis design /evaluation, diagnosis quality /standard, microarray technology, neoplasm /cancer diagnosis, neoplasm /cancer genetics
gene expression, tumor progression
SDS polyacrylamide gel electrophoresis, cell line, clinical research, fluorescent dye /probe, high throughput technology, human genetic material tag, human tissue, polymerase chain reaction, tissue /cell culture
Institution: NORTHWESTERN UNIVERSITY 750 N. Lake Shore Drive, 7TH CHICAGO, IL 60611 Fiscal Year: 2002 Department: ROBERT H LURIE COMPREHENSIVE CANCER CENTER Project Start: 01-SEP-2002 Project End: 31-AUG-2004 ICD: NATIONAL CANCER INSTITUTE IRG: ZCA1