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Abstract

Grant Number:	5R21CA085416-02
Project Title:	DETECTION OF MDS USING FISH IN PRETRANSPLANT SAMPLES

PI Information:	Name	Email	Title
	HURD, DAVID D.	dhurd@wfubmc.edu

Abstract: DESCRIPTION: (Adapted from the investigator's abstract) Therapy associated myelodysplastic syndromes (tMDS) and acute myelogenous leukemia (tAML) have been seen with increasing frequency following high dose chemotherapy and autologous hematopoietic stem cell transplantation (AHSCT). However, tMDS and tAML can also develop following the use of standard doses of chemotherapy and radiation for the treatment of patients for malignancy. Some investigators have hypothesized that the preparative regimen for transplant is the primary etiology for the post AHSCT tMDS/tAML. Other investigators have hypothesized that it is the exposure to the prior chemotherapy and/or radiation therapy that causes the cytogenetic abnormalities in the hematopoietic stem cells. These stem cells are then collected and stored and are given back to patients following the high dose therapy. While the tMDS/tAML is then expressed post AHSCT, the etiology is the prior treatment. Standard cytogenetic analysis of bone marrow samples prior to transplant has not proven to be sensitive enough to reliably detect which patients are at the highest risk for developing tMDS/tAML. However, in a pilot study using FISH, 9 of 12 patients who developed tMDS/tAML following AHSCT were found to have the same cytogenetic abnormality in the pre-transplant samples strongly suggesting that the latter hypothesis is correct. In none of the patients tested did standard cytogenetic analysis of metaphase karyotypes detect the cytogenetic abnormality. This proposal will expand the experience of using FISH to detect tMDS/tAML associated cytogenetic abnormalities to determine the sensitivity of this technique. If FISH can be shown to reliably predict which patients are at high risk for the development tMDS/tAML, these patients should be excluded from AHSCT and alternative treatment approaches could be applied.

Public Health Relevance:
This Public Health Relevance is not available.

Thesaurus Terms:
acute myelogenous leukemia, diagnosis design /evaluation, fluorescent in situ hybridization, neoplasm /cancer diagnosis, preneoplastic state
autologous transplantation, chromosome aberration, stem cell transplantation
cryopreservation, human subject, patient oriented research

Institution:	WAKE FOREST UNIVERSITY HEALTH SCIENCES
	MEDICAL CENTER BLVD
	WINSTON-SALEM, NC 27157
Fiscal Year:	2002
Department:	INTERNAL MEDICINE
Project Start:	01-MAR-2001
Project End:	29-FEB-2004
ICD:	NATIONAL CANCER INSTITUTE
IRG:	PTHB

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