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Abstract

Grant Number:	5R37CA054198-12
Project Title:	E PROTEIN AND B CELL DIFFERENTIATION

PI Information:	Name	Email	Title
	MURRE, CORNELIS	cmurre@ucsd.edu	PROFESSOR

Abstract: DESCRIPTION (adapted from investigator's abstract): The overall objective of the research proposed in this grant application is to examine the role of helix-loop-helix proteins in B-lineage development. The E2A proteins are helix-loop-helix proteins, which in B lymphocytes bind as homodimers. We have recently shown that the E2A proteins play essential roles throughout B cell development. Both E12 and E47 are required during the early stages prior to the onset of immunoglobulin (Ig) rearrangement. They have the ability to promote IgH and Ig kappa rearrangement. Finally, they are required to induce class switch recombination in activated B lineage cells. We propose to continue these studies. In particular we would investigate the relationship of E2A, EBF and Pax-5. We would examine how E2A proteins regulate Ig V(D)J recombination. We would assess the role of Id2 and Id3 in early B lineage development and examine how E2A and Id proteins are regulated during the pro-B to pre-B cell transition. We would clarify whether E12 or E47 or both are required for Ig isotype switching. Finally, we would examine how E2A protein levels are regulated in activated mature B lineage cells. Overall the dissection of the roles of E2A and Id in B cell development should help to clarify the mechanism of how HLH proteins regulate B lineage development.

Public Health Relevance:
This Public Health Relevance is not available.

Thesaurus Terms:
B lymphocyte, cell differentiation, transcription factor
chimeric protein, gene expression, gene mutation, gene rearrangement, guanine nucleotide binding protein, humoral immunity, leukocyte activation /transformation
gene targeting, laboratory mouse, transgenic animal

Institution:	UNIVERSITY OF CALIFORNIA SAN DIEGO
	9500 GILMAN DR, DEPT 0934
	LA JOLLA, CA 920930934
Fiscal Year:	2002
Department:	DIVISION OF BIOLOGICAL SCIENCES
Project Start:	01-APR-1991
Project End:	31-JAN-2006
ICD:	NATIONAL CANCER INSTITUTE
IRG:	ALY

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